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e coli 70s ribosomes  (New England Biolabs)


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    New England Biolabs e coli 70s ribosomes
    Detection of <t>E.</t> <t>coli</t> rRNA modifications by LC-MS/MS following Tth-MazF1 Cleavage. ( A ) A schematic of a Tth-MazF1 digest of a mixture of E. coli rRNA isolated from <t>70S</t> ribosomes. ( B ) Fractional sequence coverage of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. ( C ) Sequence coverage maps of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. A complete list and description of the modifications are provided in . Data are a summary of two replicate experiments.
    E Coli 70s Ribosomes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+ribosome/pmc13107127-192-6-10?v=New+England+Biolabs
    Average 95 stars, based on 111 article reviews
    e coli 70s ribosomes - by Bioz Stars, 2026-08
    95/100 stars

    Images

    1) Product Images from "Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens"

    Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkag338

    Detection of E. coli rRNA modifications by LC-MS/MS following Tth-MazF1 Cleavage. ( A ) A schematic of a Tth-MazF1 digest of a mixture of E. coli rRNA isolated from 70S ribosomes. ( B ) Fractional sequence coverage of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. ( C ) Sequence coverage maps of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. A complete list and description of the modifications are provided in . Data are a summary of two replicate experiments.
    Figure Legend Snippet: Detection of E. coli rRNA modifications by LC-MS/MS following Tth-MazF1 Cleavage. ( A ) A schematic of a Tth-MazF1 digest of a mixture of E. coli rRNA isolated from 70S ribosomes. ( B ) Fractional sequence coverage of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. ( C ) Sequence coverage maps of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. A complete list and description of the modifications are provided in . Data are a summary of two replicate experiments.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Isolation, Sequencing



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    Image Search Results


    Detection of E. coli rRNA modifications by LC-MS/MS following Tth-MazF1 Cleavage. ( A ) A schematic of a Tth-MazF1 digest of a mixture of E. coli rRNA isolated from 70S ribosomes. ( B ) Fractional sequence coverage of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. ( C ) Sequence coverage maps of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. A complete list and description of the modifications are provided in . Data are a summary of two replicate experiments.

    Journal: Nucleic Acids Research

    Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens

    doi: 10.1093/nar/gkag338

    Figure Lengend Snippet: Detection of E. coli rRNA modifications by LC-MS/MS following Tth-MazF1 Cleavage. ( A ) A schematic of a Tth-MazF1 digest of a mixture of E. coli rRNA isolated from 70S ribosomes. ( B ) Fractional sequence coverage of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. ( C ) Sequence coverage maps of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. A complete list and description of the modifications are provided in . Data are a summary of two replicate experiments.

    Article Snippet: Escherichia coli rRNA was purified from E. coli 70S ribosomes (NEB, P0763S) using the Monarch® RNA Cleanup Kit (NEB, T2050) following the manufacturer’s protocol.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Isolation, Sequencing